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However, characterizations of SHBG and ABP were hampered by the inability to purify the proteins to homogeneity. During the middle of the decade a major breakthrough was made with the development of steroid affinity columns ( Renoir and Mercier-Bodard, 1974; Rosner and Smith, 1975; Mickelson and Petra, 1975; Musto et al, anabolic steroid for fat loss. Plasma SHBG was purified to homogeneity from first human and then numerous other species.
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